All proteins are prepared in E.coli or mammalian cells, extensively purified and tested
We use them as high grade reagents for our own research
LPS free when necessary
Production scale: mg, except for STAT3 (see list)
Storage stability and shipping conditions tested only for selected proteins (so far)
Large scale production of proteins labeled with * are not yet avaiable
The approximated cost of 1mg of product (commerciale@theriaca.it)
Human Chemokines / Interleukins and related
CXCL12 (SDF1) Full protein isophorm α No tag E. coli
IL1β Full protein No tag E. coli
IL3 Full protein No tag E. coli
IL3 Receptor alpha Domain I+II+III (CD123) Extracellular part of the receptor C-term HisTag E. coli
IL3 Receptor alpha (CD123) – Domain I + II No tag E. coli
IL3 Receptor alpha (CD123) – Domain II + III C-term HisTag E. coli
SCF (Stem Cell Factor) Soluble form of the extracellular part No tag E. coli
GM-CSF Full protein No tag E. coli
TIM-3 (Human Hepatitis A Immunoglobulin V-set domain C-term HisTag E. coli
virus cellular receptor 2, HAVCR2) E. coli
HMGB1 Full protein No tag or with N-term HisTag E. coli
HMGB1 Box A BoxA of HMGB1 No tag or with N-term HisTag E. coli
HMGB1 Box B BoxB of HMGB1 N-term HisTag E. coli
Other Human Proteins
Estrogen Receptor Alpha Steroid binding domain N-term HisTag E. coli
LC3 Full protein No tag E. coli
TASK 3 Cytoplasmatic region of Potassium No tag E. coli
channel subfamily K member 9
FSH receptor* Extracellular part of the receptor C-term HisTag E. coli
Viruses
Dengue virus E protein DIII, serotype 1-2-3-4 DIII of Dengue virus E protein No tag E. coli
Zika Virus E protein DIII DIII of Zika virus E protein No tag E. coli
JC Virus VP1 Full protein without the disorder region C-term HisTag E. coli
Influenza A H1N1 M1 Protein Full protein C-term HisTag E. coli
Parvovirus VP1u Fragment of Human Parvovirus B19 VP1u C-term HisTag E. coli
Full protein of Human Parvovirus B19 VP1u C-term HisTag E. coli
SARS-CoV2 RBD (331-524; from protein id. QHD43416.1) C-term HisTag E. coli
SARS-CoV2 RBD-SD1 (319-591; from protein id. QHD43416.1) C-term HisTag Expi293
Bacterial Proteins
Apyrase from Shigella flexneri Full protein No tag or HisTag E. coli
OmpA (Outer membrane protein Omp38 Full protein N-term HisTag E. coli
from Acinetobacter baumannii)
From Campylobacter jejuni
CadF (Fibronectin-binding protein)* Full protein C-term HisTag E. coli
FlaA (Flagellin A) Full protein No tag E. coli
FliD (Flagellar hook-associated protein 2) Full protein C-term HisTag E. coli
FlpA (Fibronectin domain-containing lipoprotein) Full protein C-term HisTag E. coli
HtrA (Periplasmic serine endoprotease DegP-like)* Full protein N-term GST E. coli
From Enteropathogenic E. coli
Aap (Dispersin) Full protein C-term HisTag E. coli
BfpA (Bundle-forming pilus) Full protein N-term HisTag E. coli
EspA (Secreted protein A) Full protein C-term HisTag E. coli
Pic (Serine protease autotransporter) Different constructs HisTag E. coli
From Corynebacterium diphtheriae
DT_B fragment B fragment of Diphtheria toxin No tag E. coli
DT_R domain Receptor binding domain of Diphtheria toxin No tag E. coli
DT_T domain Translocation domain of Diphtheria toxin No tag E. coli
Mouse Proteins
STAT3 Low yield Full protein N-term GST E. coli
Servizi Personalizzati
- Custom protein design, engineering e produzione
- Design e produzione di multispecifics antibodies (e.g. bispecifics, single chain antibodies etc)
- Epitope mapping di anticorpi anti-SARS2
- Studi strutturali, in particolare di complessi anticorpo-antigene attraverso experimentally validated computational simulations
- Caratterizzazione Biofisica. SPR, DLS, NMR, CD, ITC, DSF e altro
Le tecniche sopracitate sono state usate per le nostre high impact publications in riviste come Cells, Science, Nature Medicine, PNAS, Nature Cell Biology etc.
Abbiamo anche esperienza di service contract con varie biotech companies, sia per caratterizzazione di anticorpi sia per protein design e production.
